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Three combinations were successfully tested on CAR-positive and -negative cells in duplex responses with a reference gene (REF) to concomitantly examine cell figures. All assays shown excellent specificity and reproducibility with neglectable inter-assay variations. For several three assays, almost perfect correlation amongst the two dPCRs (Axi-cel versus REF) had been seen, and the limit of detection ended up being a unitary CAR-transduced cellular correspon