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An indirect enzyme-linked immunosorbent assay (I-ELISA) based on the VP2 protein of duck hepatitis A virus type 3 (DHAV-3) was established in this study. The optimal dilutions of antigen, serum and goat anti-duck IgG conjugate were 11600 (2.23 μg/mL), 1160 and 12000, respectively. The optimal blocking buffer was 1% skim milk. The cut-off value for the method was 0.25, and the analytical sensitivity of the method was 15120. The results of specific evaluation showed that except for DHAV-1, DHAV-3 antisera did not cross-react with any other
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