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© 2021 Wiley Periodicals LLC. Basic Protocol 1 Establishment of brain tumor cell lines from patient-derived CUSA samples processing brain tumor sample from the OR to the lab Support Protocol 1 Sterilization of microsurgical tools in preparation for dissection Support Protocol 2 Collagen coating of tissue culture flasks Basic Protocol 2 Selection of tumor cells in vitro Support Protocol 3 FACS sorting tumor sample to isolate cancer cells from heterogeneous cell population.T cell activation is a fine-tuned process that involves T cell rec

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