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The aim of the present study was to evaluate the influence of sodium trimetaphosphate (TMP), associated or not with fluoride (F), on the concentrations of F, calcium (Ca), and phosphorus (P) and on the pH of mixed biofilms of Streptococcus mutans and Candida albicans, before and after exposure to sucrose. The biofilms received three treatments (72, 78, and 96 h after the beginning of their formation), at three TMP concentrations (0.25, 0.5, or 1%), with or without F at 500 ppm. Solutions containing 500 and 1,100 ppm F as well as artificial saliva were also