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001 to p0.05). The SOD and catalase activities, SIRT1 protein expression, sperm parameters in VE+HFD group were lower than of those of VE group, however, MDA content, expression of Acp53 protein, apoptosis indexes in VE+HFD group was higher than that of VE group (p0.001 to p0.05). This study revealed that voluntary exercise improved spermatogenesis, in part by decreasing the testicular oxidative stress status, apoptosis through alteration in miR-34a/SIRT1/p53 pathway. This study revealed that voluntary exercise improved sper