https://www.selleckchem.com/pr....oducts/ex229-compoun
This review offers significant and comprehensive guidance for further clinical research on PD. Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into zygotes are used for generating genetically modified pigs. However, these methods create mosaic mutations in embryos. In this study, we evaluated whether the gene editing method and embryonic stage for gene editing affect the gene editing efficiency of porcine embryos. First, we designed five guide RNAs (gRNAs) targeting the B4GALNT2 gene and evaluated mut