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The IC50 value of tacrine was calculated to be 0.43 μM, and the detection limit of diazinon was 0.22 μM. Both of these performances were much better than previous results, revealing our probe is sensitive and reversible for screening applications.We developed a new procedure for the comprehensive analysis of metabolites and enzymes involved in polyamine metabolism pathways. The procedure utilizes stable isotope-labeled polyamines and directly and precisely determines labeled products from enzymatic reactions by ESI-Q-TOF-MS. The activity