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Genes related to denitrification and nitrate reduction in N300+ST were higher than those in N300, and N300+BC significantly increased nitrification and glutamate synthesis genes. Therefore, N fertilizer application plus cotton straw-derived biochar changed the microbial community composition, increased nitrification and glutamate synthesis enzyme genes which were beneficial to the accumulation of soil N content, and improved soil N retention capacity thus to increase N fertilizer use efficiency.In this study, we investigated the seropre
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