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C substitution of the ABO gene may lead to allelic competition in the Bw11 subtype. Combined molecular and serological methods is helpful for precise blood grouping. To explore the molecular basis for an individual suspected as AwB subtype through DNA sequencing. ABO serology was carried out with the standard tube method. To identify the ABO gene haplotype, the amplicons of exon 7 were cloned and sequenced. Serological results showed that the forward typing was AwB and the reverse typing was B. Sequencing analysis revealed that the sa