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Thus, by measuring the system fluorescence intensity and exploiting FRET signal difference, UDG activity can be detected in a simple process. The detection limit is 0.087 U/mL without requiring additional signal amplification process. Besides, our developed strategy can also be used for screening the UDG inhibitors in a ratiometric fluorescence detection way.A novel approach for the online coupling of solid-phase microextraction (SPME) and liquid chromatography (LC) is introduced. An innovative Si@GO@βCD coated needle-sleeve extractant