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Multilocus sequence typing (MLST) of 39 stx2a-positive isolates collected from both countries revealed substantial diversity with 19 different sequence types. Only a few classical LEE-positive strains similar to HUSEC were found among the stx2a-positive isolates, notably a single O121H19 and an O26H11. Lineages known to include LEE-negative HUSEC were also recovered including, such as O113H21 (sequence type ST-223), O130H11 (ST-297), and O101H33 (ST-33. We conclude that E. coli encoding stx2a in cattle are ranging from strains similar t