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The key factors that degrade the MEA performance are highlighted. Innovative, emerging material concepts and development of low-PGM and PGM-free catalysts are discussed. © 2020 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim.Positively charged reversed-phase liquid chromatography was employed for the efficient preparative separation of isoquinoline alkaloids from Corydalis impatiens. Ten commercially available columns were compared for isoquinoline alkaloids analysis. While tailing, overloading, lower resolution, and buffer salts limite