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In arteries, additional technical cues derived from the pulsatile the flow of blood and force play a pivotal role in homeostasis and disease development. Currently, the nature regarding the cues from the ECM and their discussion utilizing the mechanical microenvironment in huge arteries to maintain the stability regarding the vessel wall surface are not totally understood. Here, we identified the matricellular necessary protein thrombospondin-1 (Thbs1) as an extracellul