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Leptospira-positive cultures were 100% (20/2 identified within the genus Leptospira (rrl gene) and 50% (10/2 corresponded to pathogenic species (lipL32 gene) by PCR, and 45% (9/2 were positive to at least one antiserum. None of the direct urine and water samples was positive by PCR. It is suggested that the bacteria might be transmitted in the animal-environment interface in the pig production cycle. It is suggested that the bacteria might be transmitted in the animal-environment interface in the pig production cycle. To observe the levels of
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