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experimental conditions, including darkness and passive movement. These findings offer critical insights into the processing of 3D space in the mammalian brain. Copyright © 2020 the authors.Mass spectrometry (MS)-based proteomics has great potential for overcoming the limitations of antibody-based immunoassays for antibody-independent, comprehensive, and quantitative proteomic analysis of single cells. Indeed, recent advances in nanoscale sample preparation have enabled effective processing of single cells. In particular, the concept of