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Animal welfare status is assessed today through visual evaluations requiring an on-farm visit. A convenient alternative would be to detect cow welfare status directly in milk samples, already routinely collected for milk recording. The objective of this study was to propose a novel approach to demonstrate that Fourier transform infrared (FTIR) spectroscopy can detect changes in milk composition related to cows subjected to movement restriction at the tie stall with four tie-rail configurations varying in height and position (TR1, TR2, T
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