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The newly developed technique would eliminate the need for expensive radioactive sources and thereby offers immense application in a variety of day to day experiments-both in students and research laboratories.We introduce a novel method to compute three-dimensional (3D) displacements and both in-plane and out-of-plane tractions on nominally planar transparent materials using standard epifluorescence microscopy. Despite the importance of out-of-plane components to fully understanding cell behavior, epifluorescence images are generally
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