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Live attenuated bacteria have been used as target vehicles for genetic therapy of malignant carcinoma because they can be reprogrammed by following simple genetic rules and have the ability to target tumor hypoxic region. In this research, noninvasive Escherichia coli (E. Coli) is genetically modified through the plasmid transfection to afford E. Coli(p) with overexpressed human catalase for catalyzing H2O2 into O2 in the tumor site. The produced O2 is consequently converted to cytotoxic 1O2 under near-infrared (NIR) light irradiation f
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