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The effects of ultrasonication and drying method on particle size and other product characteristics of bio-calcium powder from Asian sea bass (Lates calcarifer) backbone were investigated. Ultrasonication was performed at different amplitudes (60%, 70%, and 80%) for varying periods (15 and 30 min). Ultrasonication at higher amplitudes for a longer time reduced the powder particle size more effectively (p .05). The bio-calcium powder ultrasonicated at 70% amplitude for 15 min had the smallest particle size (3.38 µm) when compared to the

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