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To explore the correlation between microRNA (miRNA) differential expression and quality of embryo. The miRNA expression profiles of 8 blastocysts were detected by a TaqMan microRNA array, and miRNAs with a stable expression were selected. Additional blastocysts were selected, and the candidate miRNA was detected by real-time PCR. Meanwhile, chromosomal abnormalities of the embryos were detected by using next-generation sequencing, and the results were compared. The expression of mir-720, mir-372, mir-886-3p and mir-512-3p was higher than t

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