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Affinity matrix had been created by bonding aminobenzohydrazides which exhibited inhibitory activity to Sepharose-4B-L-tyrosine. HRP was isolated from crude homogenate in single-step and purification aspects had been taped as 1151 fold (data recovery of 8.5 percent) with 4-amino 3-bromo benzohydrazide, and purification factors had been taped as 166.16 fold (data recovery of 16.67 %) with 3-amino
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