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This research defines the growth and validation of a multiplex RT-PCR assay for detection and subtyping of IAV from pigs. The analytical and diagnostic specificity of the assays was 100% (94.3-100.0, CI 95%), plus the restriction of recognition was 10-3 TCID50/mL. A total of 100 samples (IAV isolates and medical specimens) had been tested, plus the virus subtype had been determined for 80 samples (80%; 71.1-86.7, CI 95%). From these, 50% were H1N1

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