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Test's specificity improved by using two distinct positivity and negativity cut-offs (7.7pg/mL and LLOD, respectively), but remained suboptimal in ICU patients (50%), as compared to haematological or other patients (93% and 90%, respectively). The classification of Wako's results as negative when
<llod, and positive when>
7.7pg/mL, could be a promising diagnostic approach to confidently rule out an IFI in both ICU and non-ICU patients. The poor specificity in the ICU setting remains a concern, due to the difficulty to interpret po

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