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7% (95%CI -1.7 - 0.3)). Breast volume increased by 69ml (IQR 58 - 134) in transdermal and 62ml (IQR 32 - 95) in oral estradiol users. This was not associated with estrone concentrations in transdermal (+ 14% (95%CI -49 - 156) per 100pmol/L increase in estrone concentration) nor oral estradiol users (+ 11% (95%CI -14 - 43)). Change in fat percentage and breast development in trans women were not associated with estrone concentrations nor with administration route. Therefore measurement of estrone concentrations does not have a place in the
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