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A 36.7 kDa extracellular mannanase was originally purified from a generally regarded as safe (GRAS) lactic acid bacteria (LA strain Lactobacillus casei HDS-01. The LC-GC/GC determined that HDS-01 mannanase contained 338 amino acid residues. This protein belonged to glycoside hydrolase (GH) 26 Family and shared high similarity (94%) but low coverage (54%) with mannanase from Bacillus licheniformis (GQ859489.1). Circular dichroism (CD) illustrated that the proportion of α + β structural elements closely related to enzyme activity and were
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