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We determined the complete mitochondrial genome (mitogenome) sequence of a Chilean freshwater crayfish, Parastacus nicoleti (Philippi, 1882). This is the first complete mitogenome sequence of a non-Australian crayfish belonging to the family Parastacidae. The complete mitogenome of P. nicoleti is 20,894 bp in length and contains 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and a putative control region (CR). In the phylogenetic analysis, freshwater crayfishes were clearly divided into two monophyly
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