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The majority of the sample had borderline deficiency; therefore, additional investigation and management are required. Our findings show a low prevalence of vitamin B12 deficiency in type 2 diabetic patients taking metformin. The majority of the sample had borderline deficiency; therefore, additional investigation and management are required. To determine the influence of caffeine on pharmacokinetics and pharmacodynamics of pioglitazone (PIO) in diabetic rats. This was a preclinical study conducted in the College of Pharmacy, Najran Uni