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Chitosan is an organic compound widely used in biomedical and agricultural fields due to its medicinal values. Chitosan is the largest biopolymer after cellulose and it is used as a food supplement as well as a primary health care product. The focus of the present study is to optimize the method for isolation and characterization of chitosan from Omani shrimp shell. The chitosan was isolated chemically from shrimp waste through the chemical processes of demineralization, deproteinization, discoloration and deacetylation. Chitosan isolat