https://www.selleckchem.com/TGF-beta.html
A simple, rapid and reliable method to quantify methotrexate (MTX) in human plasma by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established and validated in two laboratories. Sample separation was achieved on a Synergi Hydro-RP column (50 mm×2.0 mm, 2.5 μm) with a gradient elution program in 3.5 min after a simple protein precipitation with methanol (MeOH) and acetonitrile (ACN) (11). About 5 mMammonium formate aqueous solution with 0.2% formic acid and ACN were used as mobile phase with a flow rate of 0.5