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Then, induction had been achieved by including arabinose, while switching the carbon source to glycerol. This tactic allowed us to obtain an eightfold increase in recombinant HMFO titer in comparison to a reference batch fermentation in Erlenmeyer flasks utilizing terrific broth (T, typically used with arabinose-inducible strains. The optimized protocol has also been tested for expression of a structurally unrelated oxidase, EUGO, where a similar yi
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