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This study provides a fundamental approach to understand the working mechanism and topological dynamics in the self-assembled elastomers, with molecularly encoded stiffening and coloration.Adriamycin (ADM)-coated silica microspheres as a label for the sensitive detection of a cancer biomarker alpha-fetoprotein (AFP) was reported. Silica microspheres (SiO2 MSs) were employed as the carrier for the immobilization of gold nanoparticles (Au NPs), secondary antibody (Ab2) and ADM (denote ADM@Au NPs@SiO2 MS/Ab2) as labels. In the presence o