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Moreover, our framework increases the flexibility in data collection, allowing us to utilize any unpaired data at any dose levels.Previous studies have explored effects of imidacloprid and its metabolites on terrestrial species, such as bees, and indicated the importance of some active metabolites. However, the biotransformation of IMI and the toxicity of its metabolites to aquatic arthropods are largely unknown, especially the mechanisms driving species sensitivity differences and time-cumulative toxicity effects. To assess the potenti