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The co-expression network was further analyzed to underpin the regulatory function of . Finally, 25 genes were selected for qRT-PCR validation, the expression profiles of showed diverse patterns, demonstrating their potential roles in relation to stress tolerance regulation. This study reported the genome organization, evolutionary characteristics and expression profile of the bHLH family in barley, which not only provide the targets for further functional analysis, but also facilitate better understanding of the regulatory network bHLH g