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016), high TMB ( = 0.0072), and overall survival of patients ( = 0.0026). mutations associated with high TMB ( = 0.0016) and metastasis-positive lymph nodes ( = 0.027). These results suggested that and could be potential prognosis biomarkers for Chinese patients with ESCC. We also found that the number of positive lymph nodes was associated with disease-free survival. Clinical target gene analysis indicated that nearly half of Chinese ESCC patients might benefit from treatment with gene-specific target drugs. Our study revealed the ESCC