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Contamination of steroidal estrogens in the environment has raised a great public concern, and therefore, developing an effective method for removal of trace amount of environmental estrogens is necessary. In this study, two estrogen-degrading bacteria were isolated from activated sludge and were identified as strain Sphingomonas sp. AHC-F and strain Sphingobium sp. AX-B. They were capable of utilizing estrone (E1) and 17ß-estradiol (E2) as sole carbon and energy source. Cell immobilization technique was applied to these two estrogen-de