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In this study, we systematically investigated the bioaccumulation and trophic transfer of arsenic (As) in a typical semi-enclosed gulf, Daya Bay. Ten categories of organisms and environmental samples for As, δ13C, and δ15N analyses were collected from 14 sampling sites in all four seasons. The results demonstrated that As concentrations in the organisms and environmental samples were within the normal range of As levels in other uncontaminated marine ecosystems. Arsenic concentrations were generally lower in the pelagic organisms than