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02) decreased and bilirubin increased (0.21 ± 0.06 log mg/dL; P  0.01) in the CR group; no between-group differences in ALT (- 1 ± 1IU/L; P  0.99) or AST (2 ± 2IU/L; P = 0.68) were revealed. However, sex-by-treatment-by-time interactions (P  0.01) were observed, with CR (vs. control) inducing reduced ALT and GGT and increased AST in men only (P ≤ 0.02). In metabolically healthy individuals without obesity, 2years of CR improves several liver biomarkers, with potentially greater improvements in men. These data suggest that sus

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