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Investigation of defensive reactions, piglet losses, post-bleeding and recovery time of suckling piglets castrated under automated isoflurane anesthesia as well as measurements of isoflurane concentrations in ambient air and estimation of the cleaning and disinfection success of anesthesia masks. A total of 955 suckling piglets (age 4.0 ± 1.2 days; weight 2.0 ± 0.5 kg) were castrated under automated isoflurane anesthesia (PorcAnest 3000 ) after the administration of a non-steroidal anti-inflammatory drug. Reactions during the procedure were