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The appearance of MMP-9 and AQP4 also were calculated by qPCR and western blot. Afterwards, we knocked-out the MMP-9 gene using MMP-9 siRNA. AQP4 and its own gene expression , as well as the LDH launch price were calculated using ELISA, west blotting, and RT-PCR. We additionally evaluated cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C (PKC), and Ca2+/calmodulin-dependent necessary protein kinase II (CaMK II) in MMP-9 knockout

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