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According to in-hospital guidelines, the biomarker S100B is used to rule-out intracranial lesions in mild-moderate head trauma. It is currently investigated if S100B is applicable in a prehospital setting. The aim was to compare S100B values and hemolysis index in blood samples drawn and stored under simulated prehospital conditions to standardized blood samples. 30 patients undergoing craniotomy at Department of Neurosurgery, Aarhus University Hospital each had six blood samples drawn. Two samples, drawn in in-hospital standardized Beck