https://www.selleckchem.com/pr....oducts/brensocatib.h
HIV-1 produces defective mutants in the process of reproduction. The significance of the mutants has not been well investigated. The plasmids of wild type (HIV-1 ) and Env-defective (HIV-1 ) HIV-1 were co-transfected into HEK293T cells. The progeny virus was collected to infect MT4 cells. The env gene and near-full-length genome (NFLG) of HIV-1 were amplified and sequenced. The phylogenetic diversity, recombinant patterns and hotspots, and the functionality of HIV-1 Env were determined. A total of 42 env genes and 8 NFLGs were succe
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