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All replacement mutants expressing RpoB Q432insQ were resistant to RIF (MIC 1 mg/L). These results indicate that RpoB Q432insQ causes RIF resistance in M. tuberculosis.Catabolic acetolactate synthase (cALS) plays a crucial role in the quality of liquor because of its ability to catalyze the synthesis of the endogenous precursor product α-acetolactate of the aromatic compound tetramethylpyrazine (TTMP) and acetoin. However, the vulnerability of cALS to acidic conditions limits its application in the Chinese liquor brewing industry.

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