https://www.selleckchem.com/products/r428.html
Bacillus subtilis has many attributes that make it a popular host for recombinant protein production. Although its protein production ability has been enhanced through protease gene disruption, residual proteases like quality control HtrA and HtrB can limit protein yield by degrading inadequately folded proteins present during overexpression. In this study, two strategies were employed to increase production of industrial enzyme pullulanase enhancing extracellular pullulanase folding and optimizing its signal peptide. The hypothesis was th