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Digoxigenin-labeled DNA probes were generated based on a specific nucleotide sequence for B. divergens, obtained by PCR and sequencing of DNA isolates from infected Ixodes ricinus ticks from deceased cattle. ISH using these probes allowed postmortem diagnosis of B. divergens infection in routinely fixed FFPE tissues. Glycogen synthase kinase 3 (GSK-3) is a constitutively active, ubiquitous expressed ser/thr kinase that is involved in a large number of signaling pathways. GSK-3 is a key target of a remarkably large numbe