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CONCLUSIONS Millefy simplifies the examination of cellular heterogeneity in RNA transcription and processing events using scRNA-seq data. Millefy is available as an R package (https//github.com/yuifu/millefy) and as a Docker image for use with Jupyter Notebook (https//hub.docker.com/r/yuifu/datascience-notebook-millefy).BACKGROUND Buffalo milk is considered as a highly nutritious food owing to its higher contents of fatty acids (FA) and rich nutrient profile. Higher fat contents of buffalo milk make it suitable for processing to develo