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Homologous recombination of the DUR3 over-expression cassette ended up being performed at the HO locus by individual transformation for the constructed plasmid CRISPR-DUR3-gBlock-HO, generating the engineered strain N85DUR1,2/DUR3-c. Consequently, the DUR3 appearance level was significantly enhanced in the modified stress, resulting in increased utilization of urea. The brewing test showed that N85DUR1,2/DUR3-c paid off urea and EC levels by 92
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