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But, whole-cell and muscle specimens challenge the doable resolution and level of nanoscopy practices. We give attention to three-dimensional single-molecule localization microscopy and review some of the significant roadblocks and developing methods to resolving dense volumes of cells and cells during the nanoscale in three proportions. These challenges include history fluorescence, system- and sample-induced aberrations, information held by photons, along with drift correction, volume repair,

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