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© 2020 British Society for Haematology and John Wiley Sons Ltd.Singlet oxygen (1 O2 ) is widely regarded as the main cytotoxic substance that induces the biological damage for photodynamic therapy (PDT). In this study, the previously developed near-infrared (NIR) optical imaging system was optimized for fast imaging of 1 O2 luminescence. The optical imaging system enables direct imaging of 1 O2 luminescence in blood vessels within 2 s during vascular targeted PDT (V-PDT), which makes this system extremely practical for in vivo studie
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