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Objective To determine whether a smartphone-obtained image can be used to extrapolate the PCV of canine blood samples. Design Prospective study of surplus blood obtained from clinical cases. Setting University teaching hospital. Interventions Blood samples were placed on filter paper, and images were obtained with a smartphone in different environments. These results were then compared to the PCV measurements on the same samples following the World Health Organization guidelines. Measurements Sixty-nine samples were assessed and identified