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Objective The aim was to develop a novel image processing protocol for confocal laser checking microscopy (CLSM) to examine mineral distribution within erosive lesions as a function of depth. Methods Polished bovine enamel samples (n = 8 had been split into groups (8/group) with comparable mean surface microhardness (SMH) values. Samples underwent erosion (1 per cent citric acid pH3.8) for 1,5,10,15, or 30 min, with or without stirring giving 10 therapy groups in a 2*5 factorial design.
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